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The criterium of treatment effeicacy was median overall survival (OS) of the animals.The clinical Phase I study comprised 15 patients with recurrent malignant gliomas.

The animals received a standard diet and had permanent access to water.

Materials and methods: The experimental study was performed on 5 groups of rats bearing C6 glioma: untreated control, tumor resection (TR) only, TR intraoperative sonodynamic therapy (i SDT), TR intraoperative photodynamic therapy (i PDT) and TR i SPDT.

Photolon was injected intravenously shortly before TR that was followed by i SDT and i PDT.

Cells in exponential growth were harvested by EDTA/Trypsin for 5 min. The trypsinization was stopped by medium with 10% FCS and the cells centrifuged.

The pellets were then resuspended in medium without any supplement, at the concentration required by the different study groups.

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